Nucleic acid methods
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Nucleic acid methods are the techniques used to study nucleic acids (DNA and RNA).
Contents
Purification
Quantification
- Abundance in weight: spectroscopic quantification
- Absolute abundance in number: quantitative PCR
- High-throughput relative abundance: DNA microarray
- High-throughput absolute abundance: SAGE
- Size: gel electrophoresis
Synthesis
- De novo: oligonucleotide synthesis
- Amplification: PCR
Kinetics
Other
- Bisulfite sequencing
- DNA sequencing
- Expression cloning
- Fluorescence in situ hybridization
- Lab-on-a-chip
- Nucleic acid simulations
- Northern blot
- Nuclear run-on assay
- Radioactivity in biological research
- Southern blot
- Sucrose gradient centrifugation
- Several bioinformatics methods, such as RNA structure prediction
See also
References
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