List of restriction enzyme cutting sites: Ba–Bc

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Main articles: List of restriction enzyme cutting sites and Restriction enzyme
Legend of nucleobases
Code Nucleotide represented
A Adenine (A)
C Cytosine (C)
G Guanine (G)
T Thymine (T)
N A, C, G or T
M A or C
R A or G
W A or T
Y C or T
S C or G
K G or T
H A, C or T
B C, G or T
V A, C or G
D A, G or T


This article contains a list of the most studied restriction enzymes whose names start with Ba to Bc inclusive. It contains approximately 120 enzymes.

The following information is given:

  • Enzyme: Accepted name of the molecule, according to the internationally adopted nomenclature[1][2], and bibliographical references. (Further reading: see the section "Nomenclature" in the article "Restriction enzyme".)
  • PDB code: Code used to identify the structure of a protein in the PDB database of protein structures. The 3D atomic structure of a protein provides highly valuable information to understand the intimate details of its mechanism of action[3][4].
  • Source: Organism that naturally produces the enzyme.
  • Recognition sequence: Sequence of DNA recognized by the enzyme and to which it specifically binds.
  • Cut: Cutting site and DNA products of the cut. The recognition sequence and the cutting site usually match, but sometimes the cutting site can be dozens of nucleotides away from the recognition site[5][6].
  • Isoschizomers and neoschizomers: An isoschizomer is an enzyme that recognizes the same sequence as another. A neoschizomer is a special type of isoschizomer that recognizes the same sequence as another, but cuts in a different manner. A maximum number of 8-10 most common isoschizomers are indicated for every enzyme but there may be many more. Neoschizomers are shown in bold and green color font (e.g.: BamHI). When "None on date" is indicated, that means that there were no registered isoschizomers in the databases on that date with a clearly defined cutting site. Isoschizomers indicated in white font and grey background correspond to enzymes not listed in the current lists:
as in this not listed enzyme:  EcoR70I 


Whole list navigation

Restriction enzymes

Ba - Bc

Enzyme PDB code Source Recognition sequence Cut Isoschizomers
Bac36I Bacillus alcalophilus 36 5' GGNCC
3' CCNGG		 5' ---G   GNCC--- 3'
3' ---CCNG   G--- 5'		 AspS9I, AvcI, BavAII, Bce22I, Bsp1894I, Bsu54I, FmuI, NspIV
BaeI [7] Bacillus sphaericus 5' ACN4GTAYC
3' TGN4CATYG		 5' ---ACN4GTAYCN6NNNNNN   --- 3'
3' ---TGN4CATYGN6N   NNNNN--- 5'		  — None on May 2010 —
BalI [8][9] Brevibacterium albidum 5' TGGCCA
3' ACCGGT		 5' ---TGG   CCA--- 3'
3' ---ACC   GGT--- 5'
Bal228I Bacillus alcalophilus 228 5' GGNCC
3' CCNGG		 5' ---G   GNCC--- 3'
3' ---CCNG   G--- 5'		 AspS9I, AvcI, BavAII, BshKI, Bsp1894I, Bsu54I, FmuI, NspIV
BamHI [10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30] 1BAM Bacillus amyloliquefaciens H 5' GGATCC
3' CCTAGG		 5' ---G   GATCC--- 3'
3' ---CCTAG   G--- 5'		 AccEBI, AliI, ApaCI, AsiI, Bce751I, Bsp98I, Bsp4009I, BspAAIII, CelI, Nsp29132II, NspSAIV, SolI, SurI
BamNxI Bacillus amyloliquefaciens N 5' GGWCC
3' CCWGG		 5' ---G   GWCC--- 3'
3' ---CCWG   G--- 5'		 BcuAI, BsrAI, CauI, EagMI, FdiI, HgiCII, HgiJI, SinI
BanI [31][32] Bacillus aneurinolyticus 5' GGYRCC
3' CCRYGG		 5' ---G   GYRCC--- 3'
3' ---CCRYG   G--- 5'		 AccB1I, BbvBI, BspT107I, Eco64I, HgiCI, HgiHI, MspB4I, PfaAI
BanII [12][31] Bacillus aneurinolyticus 5' GRGCYC
3' CYCGRG		 5' ---GRGCY   C--- 3'
3' ---C   YCGRG--- 5'
BanIII [31] Bacillus aneurinolyticus 5' ATCGAT
3' TAGCTA		 5' ---AT   CGAT--- 3'
3' ---TAGC   TA--- 5'		 AagI, BavCI, Bsa29I, BseCI, BspDI, Bsu15I, BsuTUI, ClaI
BanAI Bacillus anthracis 5' GGCC
3' CCGG		 5' ---GG   CC--- 3'
3' ---CC   GG--- 5'
BasI Bacillus sp. 5' CCAN5TGG
3' GGTN5ACC		 5' ---CCANNNN   NTGG--- 3'
3' ---GGTN   NNNNACC--- 5'		 AccB7I, AcpII, Asp10HII, Esp1396I, PflBI, PflMI, Van91I
BauI Bacillus aquaemaris RFL1 5' CACGAG
3' GTGCTC		 5' ---C   ACGAG--- 3'
3' ---GTGCT   C--- 5'
BavI Bacillus alvei 5' CAGCTG
3' GTCGAC		 5' ---CAG   CTG--- 3'
3' ---GTC   GAC--- 5'
BavAI Bacillus alvei A 5' CAGCTG
3' GTCGAC		 5' ---CAG   CTG--- 3'
3' ---GTC   GAC--- 5'
BavAII Bacillus alvei A 5' GGNCC
3' CCNGG		 5' ---G   GNCC--- 3'
3' ---CCNG   G--- 5'		 AspS9I, AvcI, BavBII, BshKI, Bsp1894I, Bsu54I, FmuI, NspIV
BavBI Bacillus alvei B 5' CAGCTG
3' GTCGAC		 5' ---CAG   CTG--- 3'
3' ---GTC   GAC--- 5'
BavBII Bacillus alvei B 5' GGNCC
3' CCNGG		 5' ---G   GNCC--- 3'
3' ---CCNG   G--- 5'		 AspS9I, Bac36I, BavAII, BshKI, BspBII, Bsu54I, FmuI, Pde12I
BavCI Bacillus alvei C 5' ATCGAT
3' TAGCTA		 5' ---AT   CGAT--- 3'
3' ---TAGC   TA--- 5'		 AagI, BanIII, Bsa29I, BseCI, BspDI, Bsu15I, BsuTUI, ClaI
BbeI [33] Bifidobacterium breve YIT4006 5' GGCGCC
3' CCGCGG		 5' ---GGCGC   C--- 3'
3' ---C   CGCGG--- 5'
BbiII Bifidobacterium bifidum YIT4007 5' GRCGYC
3' CYGCRG		 5' ---GR   CGYC--- 3'
3' ---CYGC   RG--- 5'		 AcyI, AhaII, BbiII, HgiHII, Hin1I, Hsp92I, Msp17I, PamII
Bbi24I Bifidobacterium bifidum S-24 5' ACGCGT
3' TGCGCA		 5' ---A   CGCGT--- 3'
3' ---TGCGC   A--- 5'
BbrI Bordetella bronchiseptica 4994 5' AAGCTT
3' TTCGAA		 5' ---A   AGCTT--- 3'
3' ---TTCGA   A--- 5'
Bbr7I Bacillus brevis 7 5' GAAGAC
3' CTTCTG		 5' ---GAAGACN6N   NNNNN--- 3'
3' ---CTTCTGN6NNNNNN   --- 5'
BbrPI [34][35] Bacillus brevis 5' CACGTG
3' GTGCAC		 5' ---CAC   GTG--- 3'
3' ---GTG   CAC--- 5'		 AcvI, BcoAI, Eco72I, PmaCI, PmlI, PspCI
BbsI [36] Bacillus laterosporus 5' GAAGAC
3' CTTCTG		 5' ---GAAGACNN   NNNN--- 3'
3' ---CTTCTGNNNNNN   --- 5'
BbuI [37] Bacillus circulans 5' GCATGC
3' CGTACG		 5' ---GCATG   C--- 3'
3' ---C   GTACG--- 5'
BbvI [38][39] Bacillus brevis 5' GCAGC
3' CGTCG		 5' ---GCAGCN7N   NNNN--- 3'
3' ---CGTCGN7NNNNN   --- 5'		 AlwXI, BseKI, BseXI, Bsp423I, Bst12I, Bst71I, BstV1I
BbvII [40] Bacillus brevis 80 5' GAAGAC
3' CTTCTG		 5' ---GAAGACNN   NNNN--- 3'
3' ---CTTCTGNNNNNN   --- 5'
Bbv12I [41] Bacillus brevis 12 5' GWGCWC
3' CWCGWG		 5' ---GWGCW   C--- 3'
3' ---C   WCGWG--- 5'
Alw21I, AspHI, Bsh45I, BsiHKAI,
HgiAI,  HpyF46II,  MspV281I
Bbv16II Bacillus brevis 16 5' GAAGAC
3' CTTCTG		 5' ---GAAGACNN   NNNN--- 3'
3' ---CTTCTGNNNNNN   --- 5'
BbvAI Bacillus brevis A 5' GAAN4TTC
3' CTTN4AAG		 5' ---GAANN   NNTTC--- 3'
3' ---CTTNN   NNAAG--- 5'		 Asp700I, MroXI, PdmI, XmnI
BbvAII Bacillus brevis A 5' ATCGAT
3' TAGCTA		 5' ---AT   CGAT--- 3'
3' ---TAGC   TA--- 5'		 BavCI, Bci29I, Bli86I, BseCI, BspZEI, Bsu15I, ClaI, Rme21I
BbvAIII Bacillus brevis A 5' TCCGGA
3' AGGCCT		 5' ---T   CCGGA--- 3'
3' ---AGGCC   T--- 5'		 AccIII, Aor13HI, BlfI, BseAI, Bsp13I, BspEI, Bsu23I, Kpn2I
BbvBI Bacillus brevis B 5' GGYRCC
3' CCRYGG		 5' ---G   GYRCC--- 3'
3' ---CCRYG   G--- 5'		 BanI, BshNI, BspT107I, Eco64I, HgiCI, HgiHI, MspB4I, PfaAI
BbvCI [42] Bacillus brevis 5' CCTCAGC
3' GGAGTCG		 5' ---CC   TCAGC--- 3'
3' ---GGAGT   CG--- 5'		 AbeI
Bca77I Bacillus caldolyticus 5' WCCGGW
3' WGGCCW		 5' ---W   CCGGW--- 3'
3' ---WGGCC   W--- 5'
BccI Bacteroides caccae 5' CCATC
3' GGTAG		 5' ---CCATCNNNN   N--- 3'
3' ---GGTAGNNNNN   --- 5'
Bce4I Bacillus cereus B4 5' GCN7GC
3' CGN7CG		 5' ---GCNNNNN   NNGC--- 3'
3' ---CGNN   NNNNNCG--- 5'
Bce22I Bacillus cereus 22 5' GGNCC
3' CCNGG		 5' ---G   GNCC--- 3'
3' ---CCNG   G--- 5'		 AspS9I, Bac36I, BavAII, BshKI, BspBII, CcuI, FmuI, Pde12I
Bce83I [43] Bacillus cereus 83 5' CTTGAG
3' GAACTC		 5' ---CTTGAGN13NNN   --- 3'
3' ---GAACTCN13N   NN--- 5'
Bce243I Bacillus cereus 5' GATC
3' CTAG		 5' ---   GATC--- 3'
3' ---CTAG   --- 5'		 Bfi57I, Bsp143I, BspJI, BstMBI, CviAI, Kzo9I, NdeII, Sth368I
Bce751I Bacillus cereus 751 5' GGATCC
3' CCTAGG		 5' ---G   GATCC--- 3'
3' ---CCTAG   G--- 5'		 BamHI, Bce751I, BnaI, Bsp98I, Bsp4009I, BstI, NspSAIV, Pfl8I
BceAI Bacillus cereus 1315 5' ACGGC
3' TGCCG		 5' ---ACGGCN11N   NN--- 3'
3' ---TGCCGN11NNN   --- 5'
BceBI Bacillus cereus 1323 5' CGCG
3' GCGC		 5' ---CG   CG--- 3'
3' ---GC   GC--- 5'		 AccII, Bsh1236I, BtkI, Csp68KVI, FalII, FauBII, FnuDII, SelI, ThaI
BceCI Bacillus cereus 1195 5' GCN7GC
3' CGN7CG		 5' ---GCNNNNN   NNGC--- 3'
3' ---CGNN   NNNNNCG--- 5'
BcefI [44] Bacillus cereus fluorescens 5' ACGGC
3' TGCCG		 5' ---ACGGCN10NN   N--- 3'
3' ---TGCCGN10NNN   --- 5'
BcgI [45][46] Bacillus coagulans 5' CGAN6TGC
3' GCTN6ACG		 5' ---CGAN6TGCN9NNN   --- 3'
3' ---GCTN6ACGN9N   NN--- 5'
Bci29I Bacillus circulans 29 5' ATCGAT
3' TAGCTA		 5' ---AT   CGAT--- 3'
3' ---TAGC   TA--- 5'		 BavCI, BciBI, Bli86I, BseCI, BspZEI, Bsu15I, ClaI, Rme21I
BciBI Bacillus circulans B 5' ATCGAT
3' TAGCTA		 5' ---AT   CGAT--- 3'
3' ---TAGC   TA--- 5'		 BavCI, BcmI, Bli86I, BseCI, BspZEI, Bsu15I, ClaI, Rme21I
BciBII Bacillus circulans B 5' CCWGG
3' GGWCC		 5' ---CC   WGG--- 3'
3' ---GGW   CC--- 5'		 AjnI, ApyI, BptI, Bst1I, BstOI, BstM6I, Bst2UI, EcoRII, MvaI
BciVI [47] Bacillus circulans 5' GTATCC
3' CATAGG		 5' ---GTATCCN4NN   --- 3'
3' ---CATAGGN4N   N--- 5'		 BfuI
BclI [15][48][49] Bacillus caldolyticus 5' TGATCA
3' ACTAGT		 5' ---T   GATCA--- 3'
3' ---ACTAG   T--- 5'
AbaI, BsiQI, BspXII,
BstT7I, FbaI, Ksp22I,  ParI 
BcmI Bacillus sp. 5' ATCGAT
3' TAGCTA		 5' ---AT   CGAT--- 3'
3' ---TAGC   TA--- 5'		 BsuTUI, Bsu15I, BavCI, Bli86I, BspZEI, Rme21I, BseCI, BdiI
BcnI [50][51][52] 2ODH Bacillus centrosporus RFL1 5' CCSGG
3' GGSCC		 5' ---CC   SGG--- 3'
3' ---GGS   CC--- 5'
AhaI, AseII, AsuC2I, BpuMI, CauII,
Eco1831I, HgiS22I,Kpn49kII, NciI
BcoI [53] Bacillus coagulans SM 1 5' CYCGRG
3' GRGCYC		 5' ---C   YCGRG--- 3'
3' ---GRGCY   C--- 5'		 Ama87I, AquI, BsoBI, BstSI, Eco88I, NspSAI, OfoI, PunAI
Bco5I Bacillus coagulans 5 5' CTCTTC
3' GAGAAG		 5' ---CTCTTCN   NNN--- 3'
3' ---GAGAAGNNNN   --- 5'
Bco27I Bacillus coagulans 27 5' CCGG
3' GGCC		 5' ---C   CGG--- 3'
3' ---GGC   C--- 5'
Bco116I Bacillus coagulans 116 5' CTCTTC
3' GAGAAG		 5' ---CTCTTCN   NNN--- 3'
3' ---GAGAAGNNNN   --- 5'
Bco118I Bacillus coagulans 118 5' RCCGGY
3' YGGCCR		 5' ---R   CCGGY--- 3'
3' ---YGGCC   R--- 5'
BcoAI Bacillus coagulans AUCM B-732 5' CACGTG
3' GTGCAC		 5' ---CAC   GTG--- 3'
3' ---GTG   CAC--- 5'		 AcvI, BbrPI, Eco72I, PmaCI, PmlI, PspCI
BcoKI Bacillus coagulans 5' CTCTTC
3' GAGAAG		 5' ---CTCTTCN   NNN--- 3'
3' ---GAGAAGNNNN   --- 5'
BcuI Bacillus coagulans Vs 29-022 5' ACTAGT
3' TGATCA		 5' ---A   CTAGT--- 3'
3' ---TGATC   A--- 5'		 AhII, AclNI, SpeI
BcuAI Bacillus cereus BKM B-814 5' GGWCC
3' CCWGG		 5' ---G   GWCC--- 3'
3' ---CCWG   G--- 5'		 BamNxI, BsrAI, Csp68KI, EagMI, FssI, HgiCII, HgiJI, SinI
This list is incomplete; you can help by expanding it.

Notes

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